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KMID : 0545120130230010076
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 1 p.76 ~ p.84
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Comparison of Bacterial Community Changes in Fermenting Kimchi at Two Different Temperatures Using a Denaturing Gradient Gel Electrophoresis Analysis
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Hong Yeun
Yang Hee-Seok
Chang Hae-Choon
Kim Hae-Yeong
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Abstract
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A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique followed by sequencing of the 16S rDNA fragments eluted from the bands of interest on denaturing gradient gels was used to monitor changes in the bacterial microflora of two commercial kimchi, salted cabbage, and ingredient mix samples during 30 days of fermentation at 4oC and 10oC. Leuconostoc (Lc.) was the dominant lactic acid bacteria (LAB) over Lactobacillus (Lb.) species at 4oC. Weissella confusa was detected in the ingredient mix and also in kimchi samples throughout fermentation in both samples at 4oC and 10oC. Lc. gelidum was detected as the dominant LAB at 4oC in both samples. The temperature affected the LAB profile of kimchi by varing the pH, which was primarily caused by the temperature-dependent competition among different LAB species in kimchi. At 4oC, the sample variations in pH and titratable acidity were more conspicuous owing to the delayed growth of LAB. Temperature affected only initial decreases in pH and initial increases in viable cell counts, but affected both the initial increases and final values of titratable acidity. The initial microflora in the kimchi sample was probably determined by the microflora of the ingredient mix, not by that of the salted cabbage. The microbial distributions in the samples used in this study resembled across the different kimchi samples and the different fermentation temperatures as the numbers of LAB increased and titratable acidity decreased.
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KEYWORD
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bacterial community, PCR-DGGE, kimchi, lactic acid bacteria
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